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Journal of Dental Research
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Comparison Among a Dip-slide Test (Dentocult®), Plate Count, and Snyder Test for Estimating Number of Lactobacilli in Human Saliva

D. Birkhed

Departments of Cariology and Oral Microbiology, School of Dentistry, Malmö

S. Edwardsson

Departments of Cariology and Oral Microbiology, School of Dentistry, Malmö

H. Andersson

Department of Mathematical Statistics, University of Lund, Lund, Sweden

Saliva samples from 120 individuals were cultured by three methods: (a) Dentocult, incubated four d at 37°C or six d at room temperature, (b) SL agar technique, and (c) Snyder test. In a supplementary study with 77 subjects, and comparing only the two former methods, the incubation time with Dentocult at room temperature was prolonged to nine d. The authors also studied whether the number of lactobacilli was influenced by storage time, transport medium, and sampling time of day.

Journal of Dental Research, Vol. 60, No. 11, 1832-1841 (1981)
DOI: 10.1177/00220345810600110201
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C. Bentley, J.J. Crawford, and C.A. Broderius
Analytical and Physiological Variability of Salivary Microbial Counts
Journal of Dental Research, November 1, 1988; 67(11): 1409 - 1413.
[Abstract] [PDF]