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Journal of Dental Research
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Characterization of Osteoblast-like Cells from Fetal Rat Calvaria

B. Kadis

Southern Illinois University, School of Dental Medicine, Edwardsville, Illinois 62026

J.M. Goodson

Forsyth Dental Center, Boston, Massachusetts 02115

S. Offenbacher

Harvard School of Dental Medicine, Boston, Massachusetts 02115

J.W. Bruns

University of California San Francisco, San Francisco, California 94143

S. Seibert

Southern Illinois University, School of Dental Medicine, Edwardsville, Illinois 62026

An improved method is described for the isolation of osteoblast-like cells from fetal rat calvaria. The cells are mononuclear, 8.3µ in diameter, alkaline phosphate positive, and possess rough endoplasmic reticulum, mitochondria, Golgi, and cytoplasmic processes. Isolated bone cells synthesize collagen and cholesterol, grow to confluency in primary culture, and respond to hormonal stimulation.

Journal of Dental Research, Vol. 59, No. 11, 2006-2013 (1980)
DOI: 10.1177/00220345800590111901
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