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Journal of Dental Research
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Identification of Collagenase in Cultured Blood Mononuclear Cells

John W. Simpson

The University of Texas Dental Branch, Dental Science Institute, P.O. Box 20068, Houston, Texas 77025

B.F. Mackler

The University of Texas Dental Branch, Dental Science Institute, P.O. Box 20068, Houston, Texas 77025

Peggy O'Neill

The University of Texas Dental Branch, Dental Science Institute, P.O. Box 20068, Houston, Texas 77025

M.L. Mailman

The University of Texas Dental Branch, Dental Science Institute, P.O. Box 20068, Houston, Texas 77025

Mononuclear cells isolated from peripheral whole blood were fractionated by erythrocyte-rosetting into T cells and non-T cell subpopulations which were tested for collagenase-producing capacity. Mononuclear cells and purified T lymphocytes, as well as non-T (B) lymphocytes, produced collagenase during serum-free culture. Removal of the macrophages from the non-T cells did not affect collagenase production by lymphocytes.

Journal of Dental Research, Vol. 59, No. 1, 2-10 (1980)
DOI: 10.1177/00220345800590010301
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