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Journal of Dental Research
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Formation of Bacterionema matruchotii Protoplast

Ichiro Takazoe

Department of Microbiology, Tokyo Dental College, Misakicho, Chiyoda-ku, Tokyo, Japan

Toshiko Itoyama

Department of Microbiology, Tokyo Dental College, Misakicho, Chiyoda-ku, Tokyo, Japan

Yasunaga Kadomura

Department of Microbiology, Tokyo Dental College, Misakicho, Chiyoda-ku, Tokyo, Japan

Protoplast formation with Bacterionema matruchotii was studied turbidimetrically and microscopically. The protoplasts were inducible during culture by addition of glycine, lysozyme and Ficoll to a medium containing Tween-80 and polyethylene glycol.

Journal of Dental Research, Vol. 58, No. 7, 1709-1713 (1979)
DOI: 10.1177/00220345790580071101
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