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Journal of Dental Research
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Sources of Variability in Rat Caries Studies: Microbial Infection and Caging Procedure

G.S. Schuster

Medical College of Georgia, Augusta, Georgia 30901, USA, School of Dentistry; Institute of Dental Research, School of Dentistry, University of Alabama in Birmingham, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland

J.M. Navia

Medical College of Georgia, Augusta, Georgia 30901, USA, School of Dentistry; Institute of Dental Research, School of Dentistry, University of Alabama in Birmingham, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland

S. Amsbaugh

Medical College of Georgia, Augusta, Georgia 30901, USA, School of Dentistry; Institute of Dental Research, School of Dentistry, University of Alabama in Birmingham, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland

R.H. Larson

Medical College of Georgia, Augusta, Georgia 30901, USA, School of Dentistry; Institute of Dental Research, School of Dentistry, University of Alabama in Birmingham, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland

Two sources of variability in rat caries experiments have been investigated: infection with two S mutans strains and two types of caging. Results indicated that strain 6715 was more virulent than was strain 10449 when 24-day-old rats were orally infected with the organzsm under standardized conditions. Caging of rats (2 per cage) in stainless steel, raised bottom cages resulted in higher level of caries than in plastic tubs (3 per cage) with hardwood bedding. Infection and caging conditions are two of the factors which may contribute to variability in caries pattern and severity in experimental rats.

Journal of Dental Research, Vol. 57, No. 2, 355-360 (1978)
DOI: 10.1177/00220345780570023601
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