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Journal of Dental Research
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Microscopic Assay for the Phagocytotic-Collagenolytic Performance (PCP Index) of Human Gingival Fibroblasts in vitro

George G. Rose

Department of Internal Medicine and Periodontics, The University of Texas Health Science Center at Houston, Dental Branch, Houston, Texas 77030

Toshihiko Yajima

Department of Internal Medicine and Periodontics, The University of Texas Health Science Center at Houston, Dental Branch, Houston, Texas 77030

Charles J. Mahan

Department of Internal Medicine and Periodontics, The University of Texas Health Science Center at Houston, Dental Branch, Houston, Texas 77030

Using 16 human gingival fibroblast cell lines from patients with periodontitis, Dilantin hyperplasia, and nonpathological gingiva, a microscopic assay was developed to quantitate the cells' ability to lyse collagen substrates. The method employs tissue culture chambers with one cover slip partially coated with a thin layer of undenatured fibrillar bovine codlagen. The assay measures the relative numbers and sizes of holes in the collagen within defined regions of the cover slips effected by the phagocytotic and collagenolytic performance (PCP) of the population of fibroblasts growing on the cover slip for 5 days. The effect on the PCP index by serum, heparin, prostaglandins, and endotoxin was evaluated.

Journal of Dental Research, Vol. 57, No. 11, 1003-1015 (1978)
DOI: 10.1177/00220345780570110101
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N. Ijuhin
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Advances in Dental Research, November 1, 1988; 2(2): 209 - 214.
[Abstract] [PDF]

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T. Yajima
Collagen Remodeling in Wound Healing by Gingival Fibroblasts in Vitro
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[Abstract] [PDF]