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Journal of Dental Research
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Biochemical and Morphological Studies of Rat Submandibular Gland: III. Effects of Testosterone Treatment on Proteins of Granule-Rich Fraction

C.T. Hanks

Institute of Dental Research, Departments of Oral Pathology and Oral Histology, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109, USA

S.G. Ghakrabarti

Institute of Dental Research, Departments of Oral Pathology and Oral Histology, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109, USA

Mature adult male rats and castrated and testosterone-treated castrated adult rats were injected with pilocarpine HGL and 3-H-lysine and sacrificed sequentially over an eight-hour time period. Following homogenization and differential centrifugation, three subcellular fractions from each group of animals were analyzed by gel electrophoresis and liquid scintillation. Two proteins in the granule-rich fraction appeared in larger amounts on the densitometric scans and appeared to represent a larger proportion of newly synthesized proteins than the other proteins.

Journal of Dental Research, Vol. 56, No. 3, 239-248 (1977)
DOI: 10.1177/00220345770560030901
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