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Journal of Dental Research
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Virulence of Streptococcus mutans: An Antibiotic-Suppressed Rat Model for Studies of Pathogenesis

Suzanne M. Michalek

The Department of Microbiology and The Institute of Dental Research, The University of Alabama in Birmingham, Birmingham, Alabama 35294, USA

Jerry R. McGhee

The Department of Microbiology and The Institute of Dental Research, The University of Alabama in Birmingham, Birmingham, Alabama 35294, USA

A simple and effective method of antibiotic suppression of the total indigenous microbial flora of rat dams and their offspring has been developed. This method allowed more effective challenge and subsequent colonization of wean-ling rat pups with a test Streptococcus mutans, since S mutans 6715 was isolated from 95% of the treated rats and only 50% of the non-treated offspring following challenge with a single inoculum of this test microorganism.

Journal of Dental Research, Vol. 56, No. 3, 205-211 (1977)
DOI: 10.1177/00220345770560030301
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