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Journal of Dental Research
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Microcapsules on Streptococcus mutans Serotypes by Electron Microscopy

Ella M. Grenier

The Dental Research Institute, School of Dentistry and Departments of Epidemiology and Environmental and Industrial Health, School of Public Health, University of Michigan, Ann Arbor, Michigan 48109, USA

R.H. Gray

The Dental Research Institute, School of Dentistry and Departments of Epidemiology and Environmental and Industrial Health, School of Public Health, University of Michigan, Ann Arbor, Michigan 48109, USA

W.J. Loesche

The Dental Research Institute, School of Dentistry and Departments of Epidemiology and Environmental and Industrial Health, School of Public Health, University of Michigan, Ann Arbor, Michigan 48109, USA

W.C. Eveland

The Dental Research Institute, School of Dentistry and Departments of Epidemiology and Environmental and Industrial Health, School of Public Health, University of Michigan, Ann Arbor, Michigan 48109, USA

Extracellular microcapsules have been demonstrated on cells of most serotypes of Streptococcus mutans by electron microscopy, using bacterial strains of the various serotypes and peroxidase labeled or unlabeled immune serum. A correlation was noted between the amount of capsular substance on the strains of S mutans examined and degree of antigenicity as expressed by the indirect fluorescent antibody (FA) title. A serotype d strain was shown to lose both antigenicity as determined by the FA reaction and capsular material as seen by electron microscopy with repeated in vitro passage. When 10% unheated rabbit serum was added to the medium, antigenicity and capsular material were restored.

Journal of Dental Research, Vol. 56, No. 2, 166-176 (1977)
DOI: 10.1177/00220345770560021101
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