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Journal of Dental Research
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Electron Microscope Comparison of Freeze-Substitution and Conventional Chemical Fixation of Undecalificied Human Dentin

Morris Lamar Hicks

US Naval Support Activity, Naples, Italy, College of Dentistry, Ohio State University, Columbus, Ohio 43210, USA

J. David Brilliant

US Naval Support Activity, Naples, Italy, College of Dentistry, Ohio State University, Columbus, Ohio 43210, USA

D.W. Foreman

US Naval Support Activity, Naples, Italy, College of Dentistry, Ohio State University, Columbus, Ohio 43210, USA

Undecalcified human dentin was prepared for electron microscopy by conventional chemical fixation with alcoholic dehydration and by freeze-substitution with cryodehydration. Comparisons are reported concerning effects of different preparation methods. Morphology remained unaltered in the pre-dentin and mineralized matrices. The technique comparison permitted inferences regarding hydration states and relative amounts of protein in different areas of the odontoblast cell.

Journal of Dental Research, Vol. 55, No. 3, 400-410 (1976)
DOI: 10.1177/00220345760550031801
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