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Journal of Dental Research
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Induced Collagenolytic Activity by Electrical Stimulation of Embryonic Fibroblasts in Tissue Culture

Benjamin B. Kamrin

Department of Biology, New York University, Washington Square, New York 10003, USA

Continuous electrical stimulation (3.5 µ amp; 1 sine wave cycle per second) of tissue culture human embryonic fibroblasts in the presence of HCL demineralized bone significantly transformed the normal cells. After 8 to 12 days of stimulation, the cells lost the capacity to form sheets; the central nucleus moved peripherally and the cells acted as phagocytes; and multinuclear cells in association with bone showed osteoclastlike properties.

Journal of Dental Research, Vol. 53, No. 6, 1475-1483 (1974)
DOI: 10.1177/00220345740530063001
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