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Journal of Dental Research
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An In Vitro Assay to Measure Early Calcium Loss from Surface Enamel

G.E. White

Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA

C.L. Cooney

Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA

A.J. Sinskey

Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA

S.A. Miller

Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA

A sensitive and quantitative assay to measure the early calcium loss from surface enamel exposed to organic acids and metabolic products of cariogenic bacteria was designed. It will be useful to show the effects of metabolic products from cariogenic organisms on the surface of enamel and the effectiveness of dental therapeutic agents before animal studies.

Journal of Dental Research, Vol. 53, No. 2, 481-485 (1974)
DOI: 10.1177/00220345740530025101
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G.E. White, C.L. Cooney, A.J. Sinskey, and S.A. Miller
Continuous Culture Studies on the Growth and Physiology of Streptococcus mutans
Journal of Dental Research, March 1, 1976; 55(2): 239 - 243.
[Abstract] [PDF]