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Journal of Dental Research
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An Improved Medium for Differentiating Acidogenic Bacteria

Bernard B. Keele, jr

Institute of Dental Research and Department of Microbiology, University of Alabama, Birmingham, Alabama 35294, USA

Mary Sue Reeves

Institute of Dental Research and Department of Microbiology, University of Alabama, Birmingham, Alabama 35294, USA

Juan M. Navia

Institute of Dental Research and Department of Microbiology, University of Alabama, Birmingham, Alabama 35294, USA

A modified medium was developed in which very finely divided calcium hydroxy phosphate and bromcresol purple were used as the indicator for the presence of acid-producing colonies on agar plates. The medium allows enumeration of acidogenic bacteria in no more than 72 hours; the results are highly reproducible.

Journal of Dental Research, Vol. 52, No. 5, 1054-1055 (1973)
DOI: 10.1177/00220345730520051001
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