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Journal of Dental Research
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Synthetic Medium for Calcification of Bacterionema matruchotii

J. Ennever

The University of Texas, Dental Science Institute, Houston, Texas 77025, USA

J.J. Vogel

The University of Texas, Dental Science Institute, Houston, Texas 77025, USA

J.L. Streckfuss

The University of Texas, Dental Science Institute, Houston, Texas 77025, USA

A synthetic medium was developed for further study of intracellular calcification by Bacterionema matruchotii. The medium contained metastable calcium phosphate, six other salts, three purines, two pyrimidines, nine vitamins, pimelic and thioctic acids, vitamin-free casein hydrolysate, and glucose. The vehicle was 0.1 M N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid buffer at pH 7.4.

Journal of Dental Research, Vol. 50, No. 5, 1327-1330 (1971)
DOI: 10.1177/00220345710500054101


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