Figure 1. Emdogain enhanced MG-63 cells’ degradation of type I collagen. MG-63 cells were incubated in the presence (d, e, f) or absence (a, b, c) of 100 µg/mL Emdogain for 20 hrs and then incubated on glass coated with 25 µg/mL of quenched fluorescence substrate DQ-collagen I for an additional 4 hrs. TIMP-2 (20 µM) was incubated with Emdogain and MG-63 cells as described above (g, h, i). Degradation of type I collagen (green fluorescence) was detected by confocal microscopy (samples: excitation, 488 nm; emission, 530 nm). Pictures were taken at 40x magnification (a–i). Differential interference contrast (DIC) images are shown. These data are representative of more than 3 independent experiments.