Figure 1. Phenotypic characterization and constitutional expression of osteotropic cytokines in gingival fibroblasts. In (A), basal mRNA levels of (1)-coll.I, ALP, OC, BSP, and BMP-2 in fibroblasts isolated from nine individuals are shown. In (B), basal mRNA expressions of IL-6, IL-11, LIF, OSM, IL-1β, TNF-, TNFR I, TNFR II, and IL-1R1 in cells isolated from seven individuals are shown. Cells were cultured for 24 hrs, and mRNA levels were analyzed by semi-quantitative RT-PCR. Target gene mRNA expressions were normalized to those of GAPDH. Numbers to the left indicate the number of cycles in the RT-PCR analyses, and numbers on top represent the labeling of cell isolates from different individuals.