Figure 3. RT-PCR to evaluate HLA-DR alpha-chain, CD83, CD86, and CD163 mRNA expression levels in type I and type II HLA-DR+ cells, and CD28 mRNA in CD28+ cells in lymphocyte-rich areas and the surrounding tissues. The densities of the bands corresponding to HLA-DR alpha-chain, CD83, CD86, CD163, and CD28 mRNA were measured with the Image J software (Version 1.37v, NIH, Bethesda, MD, USA), normalized against the density of the bands for GAPDH, and described numerically.