Figure 3. The anti-c-Fms antibody arrests osteoclastogenesis in vitro. (A) Bone marrow macrophages were cultured in the presence of M-CSF (100 ng/mL) and TNF- (50 ng/mL) with increasing amounts of anti-c-Fms antibody, for 3 days. Cells were stained for TRAP activity for the identification of osteoclasts. (B) Numbers of osteoclasts generated in wells containing various amounts of anti-c-Fms antibody. The numbers of osteoclasts were counted in 4 replicates. *p < 0.05 vs. 0 ng/mL by the Mann-Whitney U-test. Scale bar: 20 µm in (A). Results are expressed as means ± SD.