Figure 3. IL-8 production by HGFs after single stimulation with IL-17, IFN-, and combined cytokine stimulation. HGF cultures were stimulated with indicated concentrations of IL-17 (A), IFN- (B), and the combined cytokines (C) for 2 days. Culture medium was used as a control. The culture supernatants were collected, and IL-8 concentrations were determined by ELISA. Data in (A) and (B) are mean ± SEM of 15 separate experiments. Each symbol in (C) represents IL-8 production of each individual culture. Horizontal lines are means of 15 separate experiments (*P < 0.05, compared with the sum of two individual cytokine stimulations).