Figure 2. Flow cytometric analysis of HLA-DR, CD40, and ICAM-1 expression by HGFs after combined stimulation with IL-17 and IFN-. HGF cultures were stimulated with indicated concentrations of IL-17 (A), IFN- (B), or combined cytokines (C) for 5 days. Culture medium was used as a control. The surface molecule expression was determined by flow cytometry. Dotted lines are isotype controls, shaded areas are unstimulated HGFs, and solid lines represent cytokine stimulation. The x-axis and the y-axis indicate the relative fluorescence intensity and cell number, respectively, and the number appearing in the upper right corner of each histogram indicates mean fluorescence intensity (MFI). Data are representative of 13 separate experiments.