Figure 1. Co-expression of EP2 with TRPV₁ in trigeminal sensory neurons. (A) Freshly isolated rat trigeminal ganglia were fixed, frozen immediately, and sectioned. The ISH-IHC was performed with a riboprobe against EP2 and an antibody against TRPV₁ protein, respectively. A representative image demonstrating the co-expression of EP2 mRNA and TRPV₁ protein is shown. The horizontal arrows denote examples of cells that expressed both. The vertical arrows in the image under the EP2-mRNA heading denote examples of cells that expressed only EP2, but not TRPV₁. Conversely, the vertical arrows in the image under the TRPV₁-protein heading denote examples of cells that expressed only TRPV₁, but not EP2. A scale bar (50 µm) is shown at the bottom left of the image. (B) Cell counting was performed on trigeminal ganglia from multiple rats (N = 3, mean ± SEM), and data are represented as percent of cells expressing the given criteria. The total numbers of neurons counted for particular criteria are shown on individual bars. (C) Cell size distribution of cells counted across all the animals was measured and presented as percent of total cells in the respective diameter range.