Figure 1. 1% AN-0128 reduced alveolar bone loss assessed histologically. We induced periodontal bone loss by placing ligatures around the right and left second maxillary molars for 7 days. The molars were treated by daily topical application of 1% AN-0128 (1% AN), vehicle alone (V), or positive control, ketorolac (K). One control group had no ligatures (NL), and another had ligatures placed but no treatment (Lig). (A) Diagram of approach to histologic analysis of bone. Briefly, the percent area defined by the boundaries, indicated by the dashed line, which consisted of bone, was measured. (B) The percent area consisting of bone was quantified with computer-assisted image analysis. (C) Bone loss was calculated from values in (B). Results represent the mean of 6 animals ± SEM. *Significantly different compared with ’ligature only’ (P < 0.05).