Figure 2. Wnt-1 strongly induced OPN expression and maintained extracellular matrix proteins. (A) Wnt-1 slightly enhanced type I alpha collagen expression by DPSCs during differentiation. (B) Wnt-1 strongly induced OPN expression in DPSCs. (C) Expression of ON was similar in both DPSC/V and DPSC/Wnt-1 cells. The total RNA from DPSC/Wnt-1 and DPSC/V cells was probed with ³²P-labeled cDNA probes for type I alpha collagen, OPN, and ON. (D) Wnt-1 partially inhibited BSP expression in DPSCs, as determined by qPCR analysis. The results represent average values from triplicate assays, and the experiments were repeated twice. OPN, osteopontin; ON, osteonectin; BSP, bone sialoprotein.