Figure 3. Supernatant of arrested oral fibroblasts enhanced the anchorage-independent growth of SAS cells. Representative illustrations of colonies after various treatments are shown in Appendix Fig. 7a. (a) Representative colonies after treatment with MMP-2 and supernatants. (b,c) Quantitation of total colonies after various treatments. (d) Quantitation of colonies after treatment with MMP-2 or supernatant of arrested oral fibroblasts and various doses of OA-Hy or LY294002. The enhancement of anchorage-independent growth was gradually reversed by a progressive increase in the doses of OA-Hy and LY294002. Each triangle represents a gradient dose of 0, 2.5, 5, and 10 µ M OA-Hy or LY294002. *p < 0.05; **p < 0.01; ***p < 0.001; in comparison with controls. ##p < 0.01; ###p < 0.001; in comparison with cells without OA-Hy or LY294002
, p < 0.05; in comparison with cells with pre-immune IgG treatment. Bars, 500 µ m. Quantitation of large colonies is shown in Appendix Figs. 7b, 7c.