Figure 4. Effects of AMPK
1 silencing on cell proliferation and AMPK1 and HIF-1 expression. RPC-C2A cells were transfected with control siRNA or siRNA against AMPK1, and incubated for 48 hrs. (A) Expression of AMPK1 protein was analyzed by Western blotting. (B) Cells were then incubated under normoxic or hypoxic conditions for an additional 24 hrs. Cell proliferation was analyzed by WST-1 reduction assay, and is expressed as percentage of control. Data represent means ± SD of three independent experiments. *p < 0.05. (C) After transfection, cells were incubated under normoxic or hypoxic conditions for an additional 4 hrs. Expression of HIF-1 protein was analyzed by Western blotting. N, normoxic conditions; H, hypoxic conditions.