Figure 3. Effects of hypoxia on expression of major AMPK subunit isoforms and HIF-1. RPC-C2A cells were incubated under hypoxic conditions for the times indicated. Protein expressions of AMPK1, β1 and 1 (A) and HIF-1 (B) were analyzed by Western blotting. Each protein level was normalized to expression of β-actin, and relative protein levels to that at 0 hr are shown. Blot values represent means ± SD of three independent experiments. *p<0.05 vs. 0 hr.