Figure 3. Histological and immunohistochemical analyses of periodontal tissue generation. (A) HE staining and immunostaining for ALP in the tissue samples given 1 mM CMC or 1 mM CMC + 1 mM Poly(P)75, with the periodontal regeneration mice model, 2 wks following surgery. Arrow: reference notch. Arrowhead: ALP-positive osteoblasts. Bar: 50 µm. (B) Comparison of bone regeneration area 1–3 wks after surgery. CMC: Carboxymethylcellulose. Error bars represent the mean ± SD of five independent analyses.