Figure 2. RT-PCR analysis. RT-PCR products were subjected to electrophoresis through 2% agarose gels with ethidium bromide staining. Gels were digitally photographed under ultraviolet transillumination. The mRNA expression levels of (A) MMP-1, (B) MMP-2, (C) MMP-3, (D) TIMP-1, (E) ADAMTS-4, (F) ADAMTS-5, (G) IL-8, and (H) G3PDH. In each panel, a representative analysis is shown above a graph indicating the normalization relative to G3PDH, as determined by Scion Image analysis. The results are expressed as the % of control (control = average of the non-loaded constructs). Data represent the mean ± SD (n = 3). Statistical significance was determined by one-way ANOVA and the LSD test for multiple comparisons between individual groups (*p < 0.05).