Figure 2. At 15 days, a cavity (c) is seen at the exposure area, partially filled by gingival overgrowth (g). After Dentonin implantation, inflammation was generally completely resolved (a). The formation of a reparative dentinal bridge (asterisk) has already started (a,b). In a few teeth, moderate inflammation is seen, together with the beginning of the repair process (asterisk) (c). Fifteen days after Dentonin implantation, x-ray microanalysis shows evidence of calcium (Ca) and phosphate (P) (d) distribution, and highlights the formation of a mineralized structure inside the pulp (asterisk). Positive anti-Dentonin staining (arrow) can still be observed around the Dentonin-coated agarose beads (e). There is a decrease in PCNA-positive cells (arrow) around Dentonin-coated agarose beads (b), compared with the staining at 8 days. Labeled cells are located near, but are not closely associated with, the surfaces of the beads (f). At this time interval, the shapes of the Dentonin-coated beads are less regular, with a few indentations, or flattening in some beads (e,f). Positive labeling can be seen for DSP in the odontoblasts, but is missing in the area where pulp repair was in progress. d = dentin (g). Positive cells for RP59 were located at the surfaces of the beads, but the overall staining was decreased compared with the staining seen at day 8 (h). In contrast, the staining for osteopontin (OPN) was enhanced at day 15, forming thick rings around the surfaces of the Dentonin-loaded beads (b) (i). Bar = 100 µm.