Figure 4. Characterization of rH58-treated enamel organ epithelial cells. Phase-contrast images showed that, after treatment with rH58 (A), enamel organ epithelial cells became rounded and larger, while the untreated control cells (B) retained their small, cobblestone-like shapes. Immunofluorescent results indicated strong positive staining for LAMP-1 (C) and amelogenin (E), but decreased immunostaining for Notch1 (G) in rH58-treated epithelial cells. The untreated control cells were stained weakly for LAMP-1 (D) and were immuno-negative for amelogenin (F), but were immunopositive for Notch1 (H). Cell nuclei were counterstained with Hoechst dye (blue). Bars = 50 µM.