Figure 1. The effect of rH58 on enamel organ epithelial cell proliferation. Exogenous rH58 at various concentrations added to enamel organ epithelial cell cultures had no significant effect on cell proliferation, as measured by an ELISA BrdU-labeling cell proliferation assay (p > 0.05, N = 3). The average RFU ± SD values obtained for cell treatments of rH58 at 0 nM, 30 nM, 90 nM, and 180 nM were 162.8 ± 9.4, 194.3 ± 18.1, 214.4 ± 61.4, and 216.6 ± 12.8, respectively.