Figure 3. Cell proliferation and alkaline phosphatase activity in primary cell cultures. Data were summarized from cell cultures derived from 6 Twist-1(+/+) and 6 Twist-1(+/–) mice at post-natal D3 and D7. Cell proliferation decreased in Twist-1(+/–) cells at both stages. This effect appeared relevant, as observed during the cell culture experiment, but it is statistically insignificant (p > 0.05 for both timepoints) (A). Alkaline phosphatase activity is about two-fold increased compared with the cells derived from wild-type molar organs at post-natal D7, which is statistically highly significant (p < 0.01) (B). Student’s t test was used to analyze the differences between groups, and all data are represented by mean ± standard deviation values.