Figure 2. CSF-1 gene expression in dental follicle cells transfected with siRNA targeting CSF-1. Results in each panel are the mean of 3 separate experiments and were compared statistically by analysis of variance and separation of means, with least significant difference at P 0.05. (Panel A) siRNA concentration effect on reduction of gene expression of CSF-l after 48 hrs of transfection. Maximum inhibition of expression is seen with 80 nM CSF-1 siRNA. (Panel B) Time-course effect of siRNA on suppression of CSF-1 gene expression, showing suppression at all times, with maximal suppression at 72 hrs. Error bars in both panels represent SDs.