Figure 3. Western blot analysis of immunoprecipitates obtained with the use of antibody to unphosphorylated JNK. Cells were treated with cyclosporin A and TNF-, and cell lysates were processed as described in Fig. 2. The blots were probed with antibodies to phosphorylated (top panels) and unphosphorylated (bottom panels) enzymes. Incubation with TNF- was for 20 min (lanes "a" and "b") or for 4 hrs (lanes "c" and "d").