Figure 2. Western blot analysis of immunoprecipitates obtained with the use of anti-p38 antibody. Human gingival fibroblasts were treated with 10 ng/mL TNF- and 10 µg/mL cyclosporin A. Cell lysates were subjected to immunoprecipitation, separated by SDS-polyacrylamide gel electrophoresis, and transferred to nitrocellulose membranes. Blots were probed with antibodies to phosphorylated (top panels) and unphosphorylated (bottom panels) enzymes. Incubation with TNF- was for 20 min (lanes "a" and "b") or for 4 hrs (lanes "c" and "d").