Figure 1. GF and cytokine concentrations in PRP supernatants over a six-day incubation period at 37°C. (A) Mean EGF concentration (pg/mL). (B) Mean IGF-1 concentration (pg/mL). (C) Mean Ang-2 concentration (pg/mL). (D) Mean IL-1β concentration (pg/mL). Final thrombin and CaCl₂•2H₂O concentrations added in PRPs were: (for condition A) 142.8 U/mL and 14.3 mg/mL; (for condition B) 28.56 U/mL and 2.86 mg/mL; and (for condition C) 5.71 U/mL and 0.57 mg/mL, respectively. Bars on histogram indicate mean ± SEM (standard error of mean). Numbers above the column represent the ratio (fold) obtained when the GF/cytokine concentration at each time was divided by the GF/cytokine concentration at t = 0 hrs. All assays were performed in triplicate for each donor, and each column represents the mean of five donors.