Figure 3. VEGF mRNA expression in oral and epidermal keratinocytes. (A) To determine relative changes in mRNA levels, we compared the densitometry values for 493-bp PCR product (VEGF₁₆₅) with β-actin expression and normalized them by setting the highest value to 100. The results are depicted graphically as the mean ± SE (n = 3). *p < 0.05 hypoxia vs. normoxia. (B) A representative gel of RT-PCR performed on RNA isolated from keratinocytes grown under normoxic and hypoxic conditions. N = normoxia, H = hypoxia.