Figure 1. CD14 expression and flow cytometric analysis of VitD₃-treated THP1 cells. (A) FACS analysis of CD14 expression on THP1 surface; arrows show CD14 expression on the surfaces of pro-monocytic THP1 cells and VitD₃-treated monocytic THP1, compared with an isotype control. The histogram is representative of results repeated on at least 5 separate occasions. (B) Population of cells gated prior to FACS analysis (R₁) with characteristics of high forward scatter (FSC) (largest cells in population) and low (< 10²) side-scatter (non-granular cells).