Figure 3. Normalized real-time RT-PCR analysis of gene expression levels by HEPM cells. The cells were cultured in triplicate, and RNA extracts for each culture were analyzed in triplicate. The data are presented as mean ± SD. (A) The cells were cultured on tissue culture plastic (TCP), commercially pure titanium (cpTi), and biomimetic apatite coating on titanium, without (HAP) and with different quantities of amelogenin incorporated into the coating. The x-axis numbers are concentrations of rM179 in µg/mL in the calcifying solution during the coating preparation. (B) The cells were cultured on tissue culture plastic in the absence (TCP) and the presence of 50 and 1000 ng/mL recombinant amelogenin (rM179) or bovine serum albumin (BSA) in the culture media.