Figure 2. Morphology of HEPM pre-osteoblast cells. Fluorescent light micrographs of cell attachment and spreading on glass coverslips (A), blank apatite coating (B), and rM179-containing coating prepared at a protein concentration of 100 µg/mL (C). The cells were immunofluorescently labeled for actin and the focal adhesion protein vinculin. Original magnification, 430X. SEM micrographs of cells cultured on cpTi (D), blank apatite coating (E), and rM179-containing coating prepared at a protein concentration of 50 µg/mL (F). Square indicates a nodule formation. Hollow arrows indicate the filopodia of the cells.