Figure 1. Characterization of amelogenin incorporation into the coating and its release kinetics into solutions. (A) Fluorescent light micrographs of the FITC labeling on the apatite coatings prepared in the absence (left) and the presence of rM179 (25 µg/mL, right). (B) Analytical HPLC profiles of the elution solutions in distilled water at different time points (2–90 hrs) and the dissolved coating solution after 90 hrs. The scale used for the elution solutions was enlarged 10 times. The samples were prepared at 50 µg/mL rM179 by superficial adsorption (left) or co-precipitation (right) processes. (C) The cumulative release profile of a sample prepared by co-precipitation process.