Figure 3. Paired immunofluorescence staining for IgG with FITC-conjugated (green) F(ab')2 fragment of anti-human IgG and galactose-deficient N-linked glycans disclosed by biotinylated PVL and AMCA-labeled avidin (blue) of gingivae from patients with advanced periodontal disease. The picture shows a representative example of inflamed periodontal disease tissue (N = 7). Most of the IgG-containing cells (green) co-stain for galactose-deficiency (blue). Arrows point to IgG-containing cells that do not stain with PVL. Bar size, 8 µm.