Journal of Dental Research -- Sánchez et al. 83 (7): 557 Figure 2

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Figure 2. Dependence of Ca-ATPase activity on pH. SR vesicles (0.1 mg/mL) from masseter (•), medial pterygoid ( ${blacktriangledown}$ ), and fast muscles ( ${blacktriangleup}$ ) were incubated at 37°C in media containing 50 mmol/L MOPS-Tris (pH 6.5–8.5) or MES-Tris (pH 5.0–6.4), 3 mmol/L ATP, 0.1 mmol/L CaCl₂, 0.1 mmol/L EGTA, 3 mmol/L MgCl₂, 100 mmol/L KCl, and 10 µmol/L calcimycin. The reactions and the Ca-ATPase activity determinations were performed as indicated in MATERIALS & METHODS. Bars, SD; n = 5.