Figure 1. Whole-blood vs. PBMC cultures after incubation with P. gingivalis LPS (10 ng/mL) for 48 hrs. Monocytes were analyzed for CD40 and CD80 expression by flow cytometry. (A) Representative histograms comparing whole-blood with PBMC cultures (n = 3). Dotted lines are isotype controls; the shaded areas are media controls; the solid lines represent cultures incubated with P. gingivalis LPS. (B) The mean MFI-fold increase of CD40 and CD80 expression on CD14⁺ monocytes of the 3 independent experiments. The data represent mean ± SE.