Figure 1. Survival and proliferation of fibroblasts on root-end filling materials. The survival and proliferation of gingival and PDL fibroblasts grown (A) on tissue culture plastic were examined fluorometrically as a measure of total DNA content within these cells. The effects of several root-end filling materials (B, Bone; C, MTA; D, Amalgam; E, HICR; F, ZOEC) were also examined. Gingival fibroblasts (triangles) and PDL fibroblasts (squares) were compared for freshly prepared materials (solid lines; solid symbols) and those thoroughly washed for 2 wks (dashed lines; open symbols). The error bars represent the standard deviation of 4 samples for each material. Plastic represents the results of 2 independent experiments, neither involving washing. ANOVA analysis was applied to all the values, and statistical significance was determined at p < 0.01. Data points represent a typical experiment that was repeated in triplicate.