Figure 1. Nodule formation by BMSC. (A) Representative wells stained with the Von-Kossa method showing more mineralized nodules in cultures treated with Emdogain (EMD) at 25 µg/mL. (B) Quantitation of the number of nodules/well (mean ± SE, N = 6/group). Only EMD at 25 µg/mL increased nodule number. ***P < 0.005 (EMD at 25 µg/mL vs. control or 100 µg/mL). (C) Quantitation of the dish area covered with mineralized nodules (mean ± SE, N = 6/group). Only EMD at 25 µg/mL increased nodule area. ***P < 0.005 (EMD at 25 µg/mL vs. control or 100 µg/mL).