Figure 3. Expression of amelogenin in the differentiation of dental epithelial progenitor cells as determined at the molecular level. (A)(a) RT-PCR. (b) Immunoblot analysis. Anti-amelogenin antibody reacted with protein bands having a molecular weight of 25 kDa. Lane 1: lower cell-density. Lane 2: higher cell-density. Lane 3: enamel organ of a rat lower incisor. (B) Classification of cells based on CK14, p75NGFR, amelogenin, ALP, Notch2, FGFR1, and FGFR2 expression patterns in lower and higher cell densities. Marker protein expression was as follows: +positive and -negative.