Figure 1. Effect of TEGDMA on GSH and GSSG levels in fibroblasts and in a cell-free system. (Panel A) Time-course of GSH and GSSG content in gingival fibroblasts treated with TEGDMA at a TD₅₀ concentration (0.3 mM). (Panel B) Time-course of depletion of GSH induced by 3 mM TEGDMA at 37°C in a cell-free system. The decrease of GSH at 1 and 3 hrs was significant (P < 0.0001 and P < 0.00001, respectively). (Panel C) Depletion of GSH induced by various concentrations of H₂O₂. (Panel D) The effect of TEGDMA on GSH in the presence or absence of GST P1. *The difference between TEGDMA+GST and TEGDMA alone was statistically significant (P < 0.0001). Error bars are standard deviation (for each group, n = 3). Data are representative of 3 separate experiments.