Figure 1. Isolation, culture-expansion, and seeding of autologous dermal fibroblasts. A 5-mm incision was made in the anterior tibia. A small piece of subcutaneous fibrous tissue (approx. 3 x 3 mm²) was removed. The isolated dermal fibroblasts were plate-cultured and expanded (A). (B) An absorbable gelatin scaffold was trimmed to 2 x 2 x 6 mm³ and immersed in the suspension of culture-expanded dermal fibroblasts at a density of 10⁷ cells/mL.