Figure 2. Localization of the biotinylated RGD peptide in alveolar tissues. ELVAX was loaded with synthetic biotinylated RGD peptide (A) or alone (B) and implanted next to the maxillary first molars in rats. The rats were killed at day 6, and the diffusion of this biotinylated peptide was assessed. Tissue sections were incubated with avidin conjugated to horseradish peroxidase (HRP) and then visualized by treatment with 3,3'-diaminobenzidine (DAB). PDL, periodontal ligament. Note the increased staining in (A), indicating the presence of the biotinylated RGD peptide.